Journal: Heliyon

Article Title: BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway

doi: 10.1016/j.heliyon.2023.e15583

Figure Lengend Snippet: Activation of AMPK signaling pathway by BSHXD. (A) P -AMPKα1 and AMPKα1 protein levels at various times, detected by Western blot analysis. (B) The relative levels of P - AMPKα1/AMPKα1. (C) PP2Aα+β, p -AMPKα1 and AMPKα1 protein levels, detected by Western blot analysis. The relative levels of (D) PP2Aα+β/β-Actin, (E)AMPKα1/β-Actin and (F) P - AMPKα1/AMPKα1. N = 3. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 compared to LPS group.

Article Snippet: The following antibodies were used: IL-1β(#31202, CST), IL-6 (ab259341, Abcam), TNF-α (ab183218, Abcam), CD86 (A1199, Abclonal), AMPKα1 (A1229, Abclonal), P -AMPKα1 (#4184, CST), PP2Aα+β (ab168350, Abcam) and β-Actin (#12262,CST), Goat Anti-Mouse (#7074, CST) and Anti-rabbit (#91196, CST).

Techniques: Activation Assay, Western Blot

Journal: Nutrients

Article Title: Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice

doi: 10.3390/nu14142929

Figure Lengend Snippet: Primary and secondary antibodies used for Western blotting.

Article Snippet: AMPKα , 1:1000 , sc-5298 , Santa , Goat-anti-rabbit , 1:5000 , ADI-SAB-300 , Enzo.

Techniques: Western Blot